Methods In49 patients with glomerulopathy, uric viral inclusion bodies were observed by staining. 方法应用染色法对49例肾小球疾病患者进行尿病毒包涵体检测。
Analysis for purification of recombinant HpaA protein of Helicobacter pylori from inclusion bodies in gel slices 幽门螺杆菌重组HpaA蛋白包涵体的切胶纯化分析
Abroad existence of Chlamydial initial and inclusion bodies in urethra mucous membrane epithelia cells of male NGU patients 男性非淋菌性尿道炎患者尿道黏膜细胞内广泛存在衣原体始体与包涵体
The virions and its inclusion bodies were observed in both nucleus and cytoplasm. 在细胞核及细胞浆内均形成圆形或椭圆形的病毒包涵体。
Optimization of the renaturation conditions for recombinant human proinsulin inclusion bodies expressed in E. 对大肠杆菌表达的重组人胰岛素原包涵体蛋白的变性复性条件进行了优化。
We determined the content of the total bacterial proteins and the proportion of recombinant porcine growth hormone ( rpGH) in inclusion bodies ( IBs) in the cultures of genetically engineered rpGH E. 测定了猪生长激素基因工程菌不同表达效率时细菌总蛋白的含量以及包涵体中重组猪生长激素占包涵体总蛋白的比值。
Comparison of two methods of purifying recombinant VacA-HpaA fusion protein inclusion bodies of Helicobacter pylori 两种纯化幽门螺旋杆菌VacA-HpaA融合蛋白包涵体方法的比较
Epithelial cells exhibited balloon-like appearance, and reticular degeneration, and inclusion bodies were found in balloon-like cells under microscope examination. 显微镜下表皮细胞呈气球状及网状变性,气球状细胞内可见包涵体。
Effect of Induction Time on Quality and Renaturation of Inclusion Bodies with Procarboxypeptidase B 诱导时机对羧肽酶原B包涵体质量和复性率影响的研究
This paper describe the formation, isolation, denaturation and renaturation of recombinant proteins as inclusion bodies in E. coli, and summarize the most efficient ways to refold recombinant proteins. 描述了大肠杆菌异源重组蛋白质的形成、制备、变性和复性,综述了国内外变性、复性的有效方法。
By the inclusion bodies dissolution, renaturation, concentration and DEAE ion exchange chromatography IFN-γ was purified. 然后,经过包涵体溶解、复性、浓缩及DEAE离子交换层析,使γ干扰素纯化。
Inclusion bodies were formed after recombinant induction by IPTG. 重组大肠杆菌经IPTG诱导形成了包涵体。
After isolation of the inclusion bodies, the optimum conditions of denaturation and renaturation were studied. 包涵体经分离洗涤后,探索了对其变性复性的最佳条件。
The inclusion bodies were identified by SDS-PAGE after denaturation and renaturation. 将包涵体变性、复性后,测定蛋白含量,并用SDS-PAGE鉴定。
This system provides a new approach for the expression of the proteins easy to form inclusion bodies. 构建的蛋白质分泌性表达的载体-宿主系统及合适的培养条件为易形成包含体的蛋白质的高效表达提供了一条新的途径。
Intracytoplasmic inclusion bodies are present in infected cells of the dermis and these pox cells. 在真皮的病变细胞和痘细胞中可见嗜酸性胞浆内包涵体。
The possible relationship among the inclusion bodies and their bearing to virus morphogenesis are discussed. 描述和讨论了包含体与细胞及病毒发育成熟的关系。
The washing procedure of inclusion bodies was improved. 完善了包涵体(Inclusionbodies,IBs)洗涤工艺。
Inclusion bodies are found in the cell plasma. 此外在细胞浆内并发现有包涵体。
Intranuclear inclusion bodies were observed in tonsils, lungs and lymph nodes. 在扁桃体、淋巴结和肺内发现嗜酸性核内包涵体。
Target protein mainly exists in the form of insoluble inclusion bodies, located in the cytoplasm. 目的蛋白主要以不溶性包涵体形式存在,定位于细胞质。
The purified inclusion bodies was dialyzed in the concentration gradient of urea to renature. 再将纯化后的包涵体置于透析袋中,在梯度浓度的尿素复性液中缓慢复性。
The Exo I was expressed and refolded from inclusion bodies. ExoⅠ在大肠杆菌中是以包涵体的形式表达的,通过复性后测定其活性。
Expressed proteins as inclusion bodies were solubilized, refolded and purified by GST affinity chromatography. 表达产物为包涵体形式,溶解和折叠后,以GST亲合层析色谱纯化。
Two recombinant proteins are not soluble form of inclusion bodies proteins. 两种重组蛋白都是以包涵体形式存在的不可溶性蛋白。
The crude inclusion bodies were washed before refolding in order to remove impurities affecting on refolding. 为消除杂质对复性的影响,在复性前,应对包涵体粗品进行洗涤。
But the experiment showed that large amounts of the protein were inactive existing in the form of inclusion bodies. 但实验发现,该蛋白大量以无活性的包涵体形式存在。
Protein was identified after breaking the main bacteria in the deposition, expressed as inclusion bodies. 经鉴定表达蛋白破菌后主要在沉淀中,为包涵体表达。
Inclusion bodies of expressed proteins were dissolved in denaturants and purified by NTA-Ni gel and refolded under suitable conditions. 用变性剂溶解包涵体蛋白,经Ni-NTA琼脂糖纯化后在适宜的条件下进行重折叠复性。